Resolution of the ATP-dependent proteolytic activity from crude reticulocyte extract into two essentially required complementing activities (adapted from Ciechanover et al.38; with permission from Elsevier/Biochem Biophys Res Commun).
Resolution of the ATP-dependent proteolytic activity from crude reticulocyte extract into two essentially required complementing activities (adapted from Ciechanover et al.38; with permission from Elsevier/Biochem Biophys Res Commun).
| Fraction | Degradation of [3H]globin (%) | |
|---|---|---|
|
| ||
| –ATP | +ATP | |
| Lysate | 1.5 | 10 |
| Fraction I | 0.0 | 0.0 |
| Fraction II | 1.5 | 2.7 |
| Fraction I and Fraction II | 1.6 | 10.6 |