The effect of PJ consumption by patients with CAS, or by diabetic patients, on their serum oxidative stress and on serum PON1 activity.

A and C: Ten patients with CAS were supplemented with PJ for a 1-year period. Blood samples were drawn from the patients before, and after 3, 6, 9, and 12 months of PJ consumption. B and D: Ten patients with type 2 diabetes mellitus were supplemented with PJ for 3 months. Blood samples were drawn from the patients before, and after PJ consumption and also from 10 healthy subjects.

A: The serum susceptibility to oxidation by 2,2′-azobis amidinopropane hydrochloride (AAPH) was determined by the lipid peroxides assay. B: Basal serum oxidative status was determined by the level of thiobarbituric acid reactive substances (TBARS). C and D: Serum PON1 arylesterase activity was measured using phenyl acetate as the substrate. Results are expressed as mean ±SD (n=10). *P<0.01 (after PJ consumption versus before treatment), ^P<0.01 (diabetic patients before versus healthy subjects), #P<0.01 (diabetic patients after PJ versus before).