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Figure 3.   Co-expression of FGL2 and Foxp3 in Treg in Tolerant Allografts

Panel A and B: Transplanted hearts were harvested from (A) rejecting mice or from (B) tolerant C3H mice at POD 100 and subsequently immunostained for Foxp3 (red) and FGL2 (green) (magnification 200×). Nuclei were visualized with DAPI (blue). Tolerant mice had significantly increased numbers of Foxp3+ Treg (white arrow). Whereas Foxp3+ Treg from tolerant mice largely expressed FGL2, Foxp3+, Treg in rejecting mice did not express FGL2. Inset shows a FGL2 Treg in a rejecting allograft and a FGL2+ Treg in a tolerant allograft (magnification 1000×). Panel C–E: Morphometric analysis of the immunostained sections was performed using a Definiens analysis assessing the (C) number of Foxp3+/μm2,(D) FGL2+/μm2, and (E) Foxp3+FGL2+/μm2. Cardiac myocytes were excluded from analysis using size exclusion. Lymphocytes were defined based on size of 10 microns or less. The morphometric analysis of heart allografts is from 6 rejecting mice, 7 tolerant mice, and 3 syngeneic mice, with 4 serial sections taken at multiple levels of the heart. Data are expressed as the mean±SEM. Statistical significance was assessed using Student’s t test. Copyright (2014) John Wiley and Sons. Used with permission from Urbanellis P, Shyu W, Khattar R, et al.49

RMMJ Rambam Maimonides Medical Journal Rambam Health Care Campus 2015 July; 6(3): e0024. ISSN: 2076-9172
Published online 2015 July 30. doi: 10.5041/RMMJ.10209