Figure 3. A 48-year-old Woman with Essential Monoclonal Gammpathy Studied Because of Episodes of Hypoglycemia
Combined agarose gel electrophoresis (A, B) and autoradiography (C, D). (A) The protein fractionation of normal serum on agarose gel. (B) The patient’s serum indicated a monoclonal IgG at the cathode. (C) A radioautograph showing a heavy band of 125I-labeled insulin bound by the monoclonal IgG. (D) The addition of unlabeled insulin markedly reduced the 125I-labeled insulin binding to monoclonal IgG. (From J Intern Med9 and reprinted with permission of John Wiley and Sons.)