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Figure 3.   After Exposure to Prolactin, GLUT1 only Partially Co-localizes with β-COP and Transferrin-Texas Red in Endosomes

Cells were maintained in prolactin-rich medium for 4 days, before they were fixed and stained with specific anti-GLUT1 or anti-β-COP primary antibodies. Some cells were exposed shortly to transferrin-Texas Red staining before fixation and exposure to anti-GLUT1. GLUT1 is shown in green, and β-COP in red after staining with FITC-conjugated and Texas Red-conjugated secondary antibodies, respectively. Transferrin stain appears in red. A, D: GLUT1 signal. B, E: β-COP and transferrin (short-term exposure) signals, respectively. C, F: Superimposed images. Partial co-localization of GLUT1 with β-COP or transferrin in endosomes appears as areas of coincident staining, giving rise to yellow signal.

RMMJ Rambam Maimonides Medical Journal Rambam Health Care Campus 2015 October; 6(4): e0038. ISSN: 2076-9172
Published online 2015 October 26. doi: 10.5041/RMMJ.10223.