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Figure 4.   After Exposure to Prolactin, GLUT1 Does not Co-localize with BODIPY-TR Ceramide

Cells were maintained in prolactin-rich medium for 4 days, before they were fixed and stained with specific anti-GLUT1 primary antibody, or stained with BODIPY-TR ceramide. GLUT1 is shown in green after staining with FITC-conjugated secondary antibody. BODIPY-TR ceramide appears in red. A, D: GLUT1 signal. B, E: BODIPY-TR ceramide signal. C, F: Superimposed image. There is little overlap of GLUT1 green signal with BODIPY-TR ceramide.

RMMJ Rambam Maimonides Medical Journal Rambam Health Care Campus 2015 October; 6(4): e0038. ISSN: 2076-9172
Published online 2015 October 26. doi: 10.5041/RMMJ.10223.