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Figure 7.   Degree of Differentiation Affects Intracellular Targeting of GLUT1

Static images of CIT3 cells in GM and at different levels of differentiation in SM. Panel A: In GM; B, C, D: In SM at different levels of differentiation. The upper left figure of each panel is GLUT1-EGFP signal. The upper right figure is staining of the same cells with BODIPY-TR ceramide (Molecular Probes, Inc., Eugene, OR, USA), which is a dye that marks the trans-Golgi in red. Living CIT3 cells were pre-incubated with 5 nmol/mL of BODIPY-TR ceramide at 4°C for 30 minutes. Lower left figure is superimposed image of the upper figures. Lower right figure is phase contrast image of the cells to show the different levels of differentiation.

RMMJ Rambam Maimonides Medical Journal Rambam Health Care Campus 2015 October; 6(4): e0038. ISSN: 2076-9172
Published online 2015 October 26. doi: 10.5041/RMMJ.10223.