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Figure 3.

Intracellular Protein Carbonylation Assay.

Left: A representative WB analysis of total intracellular proteins exposed to aldehydes. A: Air-exposed control; B: 1 μmol of acrolein (equal to 1 cigarette); C: 10 μmol of acrolein (equal to 10 cigarettes); D: 20 μmol of acetaldehyde (equal to 1 cigarette); E: 200 μmol of acetaldehyde (equal to 10 cigarettes). Right: Intracellular protein carbonyl ratio. Average densitometric analyses of three to five different WB assays of the same experiment. Reprinted from Figure 3, Panels II and III of Avezov K, et al.,49 with permission from Elsevier.

RMMJ Rambam Maimonides Medical Journal Rambam Health Care Campus 2016 July; 7(3): e0024. ISSN: 2076-9172
Published online 2016 July 28. doi: 10.5041/RMMJ.10251
Copyright: © 2016 Aizenbud et al.